phospho src tyr 416  (Cell Signaling Technology Inc)

Journal: Heliyon

Article Title: Myricetin as a promising inhibitor of platelet fibrinogen receptor in humans

doi: 10.1016/j.heliyon.2023.e20286

Figure Lengend Snippet: Regulation of myricetin on the activation of integrin α IIb β 3 and phosphorylation of integrin β 3 , Src, and Syk on a surface coated with fibrinogen. (A) Platelets (3.6 × 10 8 cells/mL) were either at (a) resting (Tyrode's solution) or preincubated with (b) 0.1% DMSO or myricetin (c, 10; d, 20 μM) and anti-PAC-1 mAb (2 μg/mL) for 3 min, after which they were stimulated with collagen (1 μg/mL) for 5 min. The number of fluorescein-labeled platelets was measured using a flow cytometer. In another experiments, washed platelets were preincubated with solvent control (0.1% DMSO) or myricetin (10 and 20 μM) and subsequently stimulated using immobilized fibrinogen (100 μg/mL). Subcellular extracts of the platelets were analyzed to determine the levels of phosphorylation of (B) integrin β 3 , (C) Src, and (D) Syk. Data are presented as the mean ± standard error of the mean ( n = 4). ** p < 0.01 and *** p < 0.001 compared with the resting (Tyrode's solution) group; # p < 0.05, ## p < 0.01, and ### p < 0.001 compared with the 0.1% DMSO + collagen group.

Article Snippet: Anti-phospho-p44/p42 extracellular signal–regulated kinase (ERK; Thr 202 /Tyr 204 ), anti-phospho- c -Jun N-terminal kinase (Thr 183 /Tyr 185 ), anti-phospho-phosphoinositide 3-kinase (PI3K) p85 (Tyr 458 )/p55 (Tyr 199 ), anti-phospho-(Ser) PKC substrate, phospho-Src family (Tyr 416 ; D49G4), and phospho-spleen tyrosine kinase (Syk; Tyr 525/526 ) pAbs were purchased from Cell Signaling (Beverly, MA, USA).

Techniques: Activation Assay, Labeling, Flow Cytometry, Solvent